Eluate
| Also Known As | Column fraction; chromatographic fraction; mobile phase fraction; collected fraction |
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What is Eluate?
The eluate is the solution that exits from a chromatographic column, containing the separated components of the original mixture dissolved in the eluent. In preparative chromatography, individual fractions of the eluate are collected sequentially. The eluate contains analytes at progressively different retention times based on their affinity for the stationary phase.
Properties & Characteristics
Uses & Applications
Safety Information
Always consult the SDS/MSDS before handling any chemical. This information is for educational purposes only.
Key Facts
Frequently Asked Questions
The eluate is the solution that exits from a chromatographic column, containing the separated components of the original mixture dissolved in the eluent. In preparative chromatography, individual fractions of the eluate are collected sequentially. The eluate contains analytes at progressively different retention times based on their affinity for the stationary phase.
Collecting purified fractions in preparative column chromatography; fraction collection in HPLC; protein purification (ion exchange, size exclusion chromatography); environmental sample analysis; pharmaceutical product isolation
Contains organic solvents (flammable, toxic) or aqueous buffers. Handle according to SDS of the eluent and analytes present. Some fractions may contain toxic intermediates or products.